Table 2.
Binding stoichiometry of yeast protein with chitooligosaccharide and betanin.
| Samples | Chitooligosaccharide/yeast protein |
Betanin/yeast protein |
||
|---|---|---|---|---|
| n | K (M−1) | n | K (M−1) | |
| Without ultrasound | 3.12 ± 0.12 | (1.24 ± 0.16) × 105 | 0.46 ± 0.02 | (3.13 ± 0.23) × 104 |
| Ultrasound (200 W, 15 min) | 4.26 ± 0.51 | (2.73 ± 0.25) × 105 | 0.61 ± 0.06 | (3.92 ± 0.10) × 104 |
| Ultrasound (400 W, 15 min) | 4.72 ± 0.26 | (2.50 ± 0.12) × 105 | 0.59 ± 0.03 | (3.78 ± 0.12) × 104 |
| Ultrasound (600 W, 15 min) | – | – | 0.23 ± 0.02 | (3.18 ± 0.18) × 104 |