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. 2024 Feb 20;103(4):103551. doi: 10.1016/j.psj.2024.103551

Figure 3.

Figure 3

Replicate study 2 ATP production rate in peripheral blood mononuclear cells (PBMC) isolated from Ross 308 chicks fed diets ± 0.03% anti-interleukin-10 before and after challenge with Eimeria maxima ± Clostridium perfringens. Chicks were inoculated with 1×108 colony forming units (CFU) of Salmonella Typhimurium on d0, 15,000 sporulated E. maxima M6 oocysts on d14, and 1×108 CFU C. perfringens on d18 and 19. Data in panels (A-F) represent the interaction effect diet and challenge at timepoints corresponding to 1, 3, 7, and 11d post-inoculation (dpi) with E. maxima or 1, 3, and 7dpi with E. maxima + C. perfringens. Stacked bars represent the mean ATP production measure measured using the Agilent XFe96 Analyzer and ATP Production rate assay from 4 (E. maxima 1 and 3dpi; E. maxima + C. perfringens 1dpi) or 6 chicks / treatment (baseline; E. maxima 7dpi/ E. maxima + C. perfringens 3dpi; E. maxima 11dpi/ E. maxima + C. perfringens 7dpi) ± SEM. Different letter labels within the same-colored bar within a timepoint represent significantly different glycolytic or mitochondrial ATP production rates, whereas different labels above an entire bar represent significantly different total ATP production, P ≤ 0.05.