Table 1.

XopJ6_CN06 and XopJ6^C409A, but not XopJ6_CN13, physically interact in planta with RRS1-R and AtWRKY22 as measured by FRET-FLIM

Donor	Acceptor	T(ns)a	Δt (ps)b	SEc	semd	Ne	E(%)f	p valueg
XopJ6CN06-CFP	–	3.8475	–	0.2541	0.0218	135
XopJ6CN06-CFP	RRS1-R-YFP	3.2694	578	0.2288	0.0293	61	15.02	7.17x10−35
XopJ6CN06-CFP	AtWKRY22-YFP	3.4824	365	0.3413	0.0314	118	9.49	3.59x10−19
XopJ6CN06-CFP	YFP	4.0041	−156	0.2572	0.0319	65	−4.07	6.81 x10−5
XopJ6C409A-CFP	–	3.7244	–	0.1384	0.0158	77
XopJ6C409A-CFP	RRS1-R-YFP	3.2726	452	0.2542	0.0328	60	12.13	2.89x10−26
XopJ6C409A-CFP	AtWKRY22-YFP	3.3641	360	0.1275	0.0180	50	9,67	3.34x10−29
XopJ6CN13-CFP	–	3,8062	–	0.2329	0.0205	129
XopJ6CN13-CFP	RRS1-R-YFP	3.6427	163	0.1748	0.0184	90	4.29	5.27x10−8
XopJ6CN13-CFP	AtWKRY22-YFP	3.8303	−24	0.2816	0.0276	50	−0.63	0.47495
XopJ6CN13-CFP	YFP	3.9187	−112	0.2516	0.0356	50	−2.96	0.00509

The statistical test used was two sided. The lifetime measurements were carried out from two to three independent expression assays performed in N. benthamiana (leaf samples were taken between 36 and 48 hours after infiltration with A. tumefaciens).

^aMean lifetime, T, in nanoseconds (ns). For each nucleus, average fluorescence decay profiles were plotted and fitted with exponential function using a non-linear square estimation procedure and the mean lifetime was calculated according to τ = Σ α_it_i² / Σ α_iti with I(t) = Σ α_i e^-t/ti.

^bΔt=τD – τDA (in ps).

^cStandard error.

^dStandard error of the mean.

^eTotal number of measured nuclei.

^f% FRET efficiency: E=1 – (τDA/τD).

^gp value of the difference between the donor lifetimes in the absence and in the presence of acceptor (Student’s t test).