Figure 7.

Ube2d3 is highly expressed in OGD-treated AC16 cells. (A) WB detection of Ube2d3 expression in cardiomyocytes AC16. (n=3) (B) qRT-PCR detection of Ube2d3 expression in cardiomyocytes AC16. (n=3, ***P<0.001) (C) MTT detection of cell activity. (n=3, **P<0.01) (D) Quantification of cytotoxicity by measuring the activity of LDH released into the culture medium from cells with ruptured plasma membranes. (n=3, ***P<0.001) (E) TUNEL staining for cell death detection, with red fluorescence indicating apoptotic cells. Under the action of TdT Enzyme, fluorescein labeled dUTP can be linked to the 3 ‘-OH end of broken DNA in apoptotic cells, showing red fluorescence. Scale bar: 100 μm, respectively. (n=3) (F) WB detection of the expression of FDX1 and SLC31A1 in cardiomyocytes AC16. (n=3) (G) WB detection of the lipoylation level of Dlat in cardiomyocytes AC16. (n=3) (H) Immunofluorescence staining showing Dlat oligomerization in cardiomyocytes. Scale bar: 100 μm, respectively. (n=3) Statistical data in this figure are presented as mean values ± SD. Statistical significance was determined by Two-tailed t test.