Table 1.
Enriched proteins identified in 2K, 10K and 100K EV fractions.
| Enriched Fraction | Protein name | Accession Number (UniProt) | Molecular Weight |
|---|---|---|---|
| 2K | Tubulin alpha chain | E9AGK7_LEIIN | 54 kDa |
| Paraflagellar rod protein 1D | E9AE36_LEIMA | 69 kDa | |
| ATP-dependent RNA helicase | A4I7K4_LEIIN | 67 kDa | |
| Tubulin beta chain | F8QV42_LEIDO | 50 kDa | |
| Proline oxidase, mitochondrial-like protein | Q4Q933_LEIMA | 64 kDa | |
| Dynein heavy chain | Q4Q9Z6_LEIMA | 535 kDa | |
| Tubulin beta chain | Q4QI42_LEIMA | 50 kDa | |
| Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | Q4QAG8_LEIMA | 67 kDa | |
| Dynein heavy chain | Q4QFY9_LEIMA | 529 kDa | |
| Pyruvate dehydrogenase E1 component alpha subunit | Q4QDQ1_LEIMA | 43 kDa | |
| 4-coumarate:coa ligase-like protein | Q4QDB5_LEIMA | 66 kDa | |
| ATP-dependent DEAD-box RNA helicase | E9AEL4_LEIMA | 46 kDa | |
| Voltage-dependent anion-selective channel | E9ACB9_LEIMA | 30 kDa | |
| Thiolase protein-like protein | Q4Q698_LEIMA | 47 kDa | |
| 2-oxoglutarate dehydrogenase E1 component | Q4Q171_LEIMA | 111 kDa | |
| Succinate–coa ligase [ADP-forming] subunit alpha, mitochondrial | A4I1R2_LEIIN | 31 kDa | |
| Malic enzyme | Q4QAQ6_LEIMA | 63 kDa | |
| Succinyl-coa:3-ketoacid-coenzyme A transferase | Q4Q3V3_LEIMA | 53 kDa | |
| 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial | Q4Q0M6_LEIMA | 32 kDa | |
| Acyl-coa dehydrogenase | Q4Q812_LEIMA | 69 kDa | |
| Possible 3-ketoacyl-coa thiolase | Q9U0V9_LEIMA | 47 kDa | |
| Calpain-like cysteine peptidase | E9AD26_LEIMA | 521 kDa | |
| Polyadenylate-binding protein | A4IBV3_LEIIN | 65 kDa | |
| 60S ribosomal protein l10a | Q4QDX9_LEIMA | 25 kDa | |
| Beta tubulin (Fragment) | Q1PCJ8_NEODS | 13 kDa | |
| RNA binding protein | Q4Q5K7_LEIMA | 25 kDa | |
| 60S ribosomal protein L9 | Q4Q6V5_LEIMA | 22 kDa | |
| 40S ribosomal protein S15A | A4HV26_LEIIN | 15 kDa | |
| Elongation factor Tu | Q4QDW8_LEIMA | 52 kDa | |
| Axoneme central apparatus protein | E9AUS9_LEIMU | 55 kDa | |
| ATP synthase, epsilon chain | Q4Q6S8_LEIMA | 20 kDa | |
| Paraflagellar rod component par4 | Q4QJJ9_LEIMA | 68 kDa | |
| ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative | E9B7L5_LEIDB | 91 kDa | |
| Kinetoplast-associated protein-like protein | E9AD01_LEIMA | 79 kDa | |
| 40S ribosomal protein S16 | Q4Q9A5_LEIMA | 17 kDa | |
| 40S ribosomal protein S19 protein | A4IA55_LEIIN | 20 kDa | |
| Trifunctional enzyme alpha subunit, mitochondrial-like protein | Q4Q939_LEIMA | 79 kDa | |
| Leucine-rich repeat protein | Q4QHX1_LEIMA | 106 kDa | |
| Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex | Q4Q1F5_LEIMA | 49 kDa | |
|
Phosphodiesterase |
Q6S997_LEIMA |
103 kDa |
|
| Nucleolar protein | Q4QHJ7_LEIMA | 53 kDa | |
| V-type proton atpase subunit a | Q4QAY7_LEIMA | 88 kDa | |
| Histone H4 | Q4QJ78_LEIMA | 11 kDa | |
| Histone H2B | Q4QDL6_LEIMA | 12 kDa | |
| Transcription factor-like protein | E9ADK4_LEIMA | 115 kDa | |
| Dynein heavy chain | Q4Q992_LEIMA | 458 kDa | |
| Heat shock protein | Q4Q3U8_LEIMA | 72 kDa | |
| Dynein heavy chain | Q4Q2F4_LEIMA | 529 kDa | |
| RNA helicase | A4HZF8_LEIIN | 59 kDa | |
| Glycoprotein 96-92 | Q4Q843_LEIMA | 87 kDa | |
| Regulatory subunit of protein kinase a-like protein | Q4Q2R2_LEIMA | 72 kDa | |
| Paraflagellar rod component | Q4QHP3_LEIMA | 68 kDa | |
| ATP-dependent DEAD/H RNA helicase | Q4QIQ9_LEIMA | 64 kDa | |
| Histone H2A | Q4QCA4_LEIMA | 14 kDa | |
| Transmembrane 9 superfamily member | Q4Q2H8_LEIMA | 71 kDa | |
| Splicing factor ptsr1-like protein | Q4QIK0_LEIMA | 42 kDa | |
| Dynein heavy chain | Q4Q8K9_LEIMA | 478 kDa | |
| Dihydrolipoamide acetyltransferase | Q4QCG0_LEIMA | 40 kDa | |
| Nucleolar RNA-binding protein | A4HTC3_LEIIN | 22 kDa | |
| Phosphodiesterase | Q6S996_LEIMA | 104 kDa | |
| Dynein heavy chain | Q4Q7X4_LEIMA | 474 kDa | |
| Flagellar radial spoke protein | Q4QGB1_LEIMA | 66 kDa | |
| Isovaleryl-coa dehydrogenase | E9AD70_LEIMA | 45 kDa | |
| Aromatic amino acid hydroxylase-like | Q6WRI4_LEIMA | 51 kDa | |
| Poly-zinc finger protein 2 | Q4Q1R1_LEIMA | 15 kDa | |
| Chaperonin HSP60/CNP60 | Q4Q711_LEIMA | 58 kDa | |
| 4-coumarate:coa ligase-like protein | Q4QDB7_LEIMA | 66 kDa | |
| Branched-chain amino acid aminotransferase | E9ADI3_LEIMA | 44 kDa | |
| Dynein | Q4Q5H6_LEIMA | 68 kDa | |
| Acyl-coa dehydrogenase | E9AF97_LEIMA | 45 kDa | |
| Histone H3 variant | Q4QDF8_LEIMA | 16 kDa | |
| Electron transfer flavoprotein-ubiquinone oxidoreductase | Q4QIN2_LEIMA | 63 kDa | |
| Mitochondrial RNA binding protein | Q4QB87_LEIMA | 40 kDa | |
| Heat shock protein-like protein | Q4Q584_LEIMA | 36 kDa | |
| Histone H3 | H3_LEIIN | 15 kDa | |
| 3-hydroxy-3-methylglutaryl coenzyme A reductase | A4I602_LEIIN | 46 kDa | |
| Beta tubulin (Fragment) | Q1PCK0_CRIFA | 14 kDa | |
| Beta-tubulin | A4HLD6_LEIBR | 21 kDa | |
| RNA-binding protein | E9AHW1_LEIIN | 30 kDa | |
| Citrate synthase | Q4QDX2_LEIMA | 50 kDa | |
| 10K | Inosine-5’-monophosphate dehydrogenase | Q4QD53_LEIMA | 56 kDa |
| GMP reductase | GMPR_LEIMA | 52 kDa | |
| Glycerol kinase, glycosomal | E9AFD2_LEIMA | 56 kDa | |
| Mevalonate kinase | Q4Q6K7_LEIMA | 35 kDa | |
| D-lactate dehydrogenase-like protein | E9ADI2_LEIMA | 54 kDa | |
| Arginase | A0A145YEM0_LEIMA | 36 kDa | |
| Ribokinase | RBSK_LEIMA | 35 kDa | |
| Aldose 1-epimerase-like protein | Q4Q2K6_LEIMA | 33 kDa | |
| 100K | Plasma membrane atpase | Q4QDN7_LEIMA | 107 kDa |
| Long chain fatty Acyl coa synthetase | E9ACH0_LEIMA | 79 kDa | |
| Adenosylhomocysteinase | Q4Q124_LEIMA | 48 kDa | |
| Phosphoglycerate kinase | Q4QD33_LEIMA | 45 kDa | |
| Glucose transporter, lmgt2 | Q4Q0D1_LEIMA | 61 kDa | |
| Long-chain-fatty-acid-coa ligase | E9AC43_LEIMA | 78 kDa | |
| Thimet oligopeptidase | Q4Q937_LEIMA | 77 kDa | |
| Aminopeptidase P | E9AF59_LEIMA | 54 kDa | |
| Inosine-guanosine transporter | Q4Q1M9_LEIMA | 54 kDa | |
| 5-methyltetrahydropteroyltriglutamate-homocystein e S-methyltransferase | Q4Q6R3_LEIMA | 86 kDa | |
| Calcium motive p-type atpase | E9AF31_LEIMA | 122 kDa | |
| Vacuolar protein sorting-associated protein 4 | E9AEB2_LEIMA | 50 kDa | |
| Metallo-peptidase, Clan MA(E), Family M32 | Q4Q3T3_LEIMA | 57 kDa | |
| 60S ribosomal protein L17, putative | A0A088RXP1_9TRYP | 19 kDa | |
| 6-phosphogluconate dehydrogenase, decarboxylating (Fragment) | Q9NGR0_LEIMA | 52 kDa | |
| Ef-hand protein 5 | A4HU12_LEIIN | 21 kDa | |
| Phospholipid-transporting atpase | Q4QG01_LEIMA | 124 kDa | |
| Dipeptidyl peptidase 3 | Q4QJA6_LEIMA | 76 kDa | |
| Pyridoxal kinase, putative | E9BLM4_LEIDB | 33 kDa | |
| Amino acid permease | Q4Q682_LEIMA | 64 kDa | |
| Cystathionine beta-synthase | Q4QEG9_LEIMA | 39 kDa | |
| Phosphomannomutase | Q4Q1M7_LEIMA | 28 kDa | |
| Dipeptylcarboxypeptidase | E9ACE8_LEIMA | 77 kDa | |
| Peptidase M20/M25/M40 | Q4Q426_LEIMA | 52 kDa | |
| ATP-binding cassette protein subfamily A,member 8 | E9AD74_LEIMA | 202 kDa | |
| Amino acid permease | Q4QBX3_LEIMA | 54 kDa | |
| Conserved SNF-7-like protein | E9AEN3_LEIMA | 26 kDa | |
| Surface antigen protein | Q4QGL8_LEIMA | 65 kDa | |
| Sucrose-phosphate synthase-like protein | Q4QES5_LEIMA | 52 kDa | |
| Folate/biopterin transporter | Q4QHH9_LEIMA | 77 kDa | |
| Arginyl-trna synthetase | E9ADB2_LEIMA | 78 kDa | |
| Kinesin-like protein | A4HY48_LEIIN | 94 kDa | |
| Amino acid transporter aatp11 | Q4Q6M9_LEIMA | 52 kDa |
Differential expression was determined using Scaffold Software, with enrichment defined as peptide counts greater than 2 standard deviations from the experiment mean (p ≤ 0.05, ANOVA). Uncharacterized proteins have been excluded from this table for clarity (see Data Sheet 2 for exhaustive list).