Figure 2.

Comparison of gDNA-PCR MRD and FCM-MRD. The plots are partitioned into four quadrants by the clinically relevant threshold of 0.1% (vertical and horizontal lines). Each symbol represents one MRD estimate. Values that are MRD-positive or MRD-negative considering the cut off of 0.1% using both methodologies are considered concordant (green dots), whereas PCR-MRD^neg/FCM-MRD^pos and PCR-MRD^pos/FCM-MRD^neg values are considered discordant (red dots). In addition, dashed lines above/below the x=y line mark the range of variance according to Dworzak et al.,³⁵ i.e. between 3x larger or smaller till 1/3 of the x=y value. Statistics performed using Graph-Pad Prism. gDNA: genomic DNA; PCR: polymerase chain reaction; MRD: measurable residual disease; FCM: flow cytometry.