Stage 1. Day 2–4 medium
| Reagent | Final concentration | Amount |
|---|---|---|
| Hematopoietic Differentiation Medium (HDM) | N/A | 999.6 μL |
| BMP4 (50 ng/μL) | 20 ng/mL | 0.4 μL |
| Total | N/A | 1 mL |
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