Stage 2. Day 4–6 medium
| Reagent | Final concentration | Amount |
|---|---|---|
| Hematopoietic Differentiation Medium (HDM) | N/A | 997 μL |
| VEGF (20 ng/μL) | 40 ng/mL | 2 μL |
| SCF (50 ng/μL) | 50 ng/mL | 1 μL |
| Total | N/A | 1 mL |
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