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. 2024 Feb 28;5(1):102925. doi: 10.1016/j.xpro.2024.102925

Stage 4. Macrophage Differentiation and Expansion medium (MDEM)

Reagent Final concentration Amount
Basal 2 Medium (RPMI 1640 Medium+10% FBS) N/A 999 μL
M-CSF (100 ng/μL) 100 ng/mL 1 μL
Total N/A 1 mL