REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PE mouse anti-human CD31 (1:500)	BD Biosciences	555446
FITC mouse anti-human CD34 (1:500)	BD Biosciences	555821
APC mouse anti-human CD45 (1:500)	BD Biosciences	555485
APC mouse anti-human CD43 (1:500)	BD Biosciences	560198
7-Amino-actinomycin D (7-AAD) (1:500)	BD Biosciences	559925
APC anti-mouse/human CD11b antibody (1:500)	BioLegend	101212
APC anti-human CD14 antibody (1:500)	BioLegend	325608
FITC anti-human CD40 antibody (1:500)	BioLegend	334306
APC anti-human CD80 antibody (1:500)	BioLegend	305220
PE anti-human CD163 antibody (1:500)	BioLegend	333606
APC anti-human CD206 (MMR) antibody (1:500)	BioLegend	321110
Chemicals, peptides, and recombinant proteins
Vitronectin XF	STEMCELL Technologies	07180
Cell Adhere dilution buffer	STEMCELL Technologies	07183
TeSR-E8	STEMCELL Technologies	05990
STEMdiff APEL 2 medium	STEMCELL Technologies	28995
KnockOut DMEM	Gibco	10829018
RPMI 1640 medium	Gibco	22400089
Fetal bovine serum (FBS)	HyClone	SH30071
Y-27632 dihydrochloride	Tocris	1254
100X Insulin-Transferrin-Selenium-X (ITS-X) supplement	Gibco	51500056
Recombinant human BMP4 protein	R&D	314-BP
Recombinant human VEGF 165 protein	R&D	293-VE
Recombinant human SCF protein	R&D	255-SC
Recombinant human Thrombopoietin (NS0-expressed) protein	R&D	288-TPN
Recombinant human IL-3 protein	R&D	203-IL
Recombinant human IL-6 protein	R&D	206-IL
Recombinant human Flt-3 ligand/FLT3L protein	R&D	308-FK
Recombinant human M-CSF	PeproTech	300-25
Lipopolysaccharides	Sigma	L2630
Human IFN-γ	Miltenyi Biotec	130-096-872
Human IL-4	Miltenyi Biotec	130-095-373
Human IL-13	Miltenyi Biotec	130-112-409
Latex beads, carboxylate-modified polystyrene, fluorescent yellow-green	Sigma-Aldrich	L4530-1ML
Experimental models: Cell lines
CMC hiPSC lines	Korea National Stem Cell Bank	CMC-003/009/011
iPS-NT4-S1 hiPSC lines	CHA University	N/A
Oligonucleotides
Primer: GAPDH forward	Bioneer	TGCACCACCAACTGCTTAGC
Primer: GAPDH reverse	Bioneer	GGCATGGACTGTGGTCATGAG
Primer: T forward	Bioneer	ATGAGCCTCGAATCCACATAGT-
Primer: T reverse	Bioneer	TCCTCGTTCTGATAAGCAGTCA
Primer: MIXL1 forward	Bioneer	GGATCCAGGTATGGTTCCAG
Primer: MIXL1 reverse	Bioneer	GGAGCACAGTGGTTGAGGAT
Software and algorithms
GraphPad Prism 10.0	GraphPad Software	https://www.graphpad.com/
FlowJo software	Tree Star	https://www.flowjo.com/
Other
35 mm cell culture dish	SPL	11035
6-well clear multiple well plates	Corning	3516
70 μm cell strainer	SPL	93070
Dissection microscope	Olympus	CKX53