Stage 2 Cytokines

Reagent	Final concentration	Reconstitution
VEGF	40 ng/mL	10 μg in 0.5 mL (20 ng/μL)
SCF	50 ng/mL	50 μg in 2 mL (25 ng/μL)

Aliquot 50 μL into a 1.5 mL microcentrifuge tube and store at −80°C for a maximum of 7 months. Limit freezing and thawing cycles.