Stage 3 Cytokines

Reagent	Final concentration	Reconstitution
SCF	50 ng/mL	50 μg in 2 mL (25 ng/μL)
TPO	10 ng/mL	25 μg in 2.5 mL (10 ng/μL)
IL-3	50 ng/mL	50 μg in 2 mL (25 ng/μL)
IL-6	50 ng/mL	50 μg in 2 mL (25 ng/μL)
Flt-3L	50 ng/mL	25 μg in 1 mL (25 ng/μL)

Aliquot 50 μL into a 1.5 mL microcentrifuge tube and store at −80°C for a maximum of 7 months. Limit freezing and thawing cycles.