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Stage 4-1 Cytokines
| Reagent | Final concentration | Reconstitution |
|---|---|---|
| LPS | 100 ng/mL | 10 mg in 10 mL (1 mg/mL) |
| IFN-γ | 20 ng/mL | 20 μg in 1 mL (20 ng/μL) |
| IL-4 | 20 ng/mL | 20 μg in 1 mL (20 ng/μL) |
| IL-13 | 20 ng/mL | 20 μg in 1 mL (20 ng/μL) |
Aliquot 50 μL into a 1.5 mL microcentrifuge tube and store at −80°C for a maximum of 7 months. Limit freezing and thawing cycles.
