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| Method | Vapor diffusion |
| Plate type | Sitting drop |
| Temperature (K) | 293 |
| Protein concentration (mg ml−1) | 4 |
| Buffer composition of protein solution | 20 mM Tris–HCl pH 7.5, 250 mM NaCl, 1 mM MgCl2, 1 mM DTT, 5% glycerol |
| Composition of reservoir solution | 90 mM Tris–HCl pH 8.5, 4.77%(w/v) PEG 4000, 360 mM CaCl2, 4.8%(v/v) 1-propanol |
| Volume of drop (µl) | 1.0 |
| Volume of reservoir (µl) | 500 |
