| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
Sarstedt 24-well plates |
Sarstedt 24-well plates |
| Temperature (K) |
291 |
283 |
| Protein concentration (mg ml−1) |
24 |
20 |
| Buffer composition of protein solution |
5 mM HEPES, 50 mM NaCl, 0.3 mM TCEP pH 7.8 |
20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 1 mM β-ME pH 7.8 |
| Composition of reservoir solution |
0.1 M CAPSO pH 9.3, 0.2 M NaCl, 1.5% glycerol, 0.03 M glycyl-glycyl-glycine, 7.5% PEG 8000 |
0.1 M Tris–HCl pH 8.5, 0.5 M NaCl, 0.25%(w/v) n-octyl-β-D-glucopyranoside, 9% PEG 4000 |
| Volume and drop ratio |
2 µl (1 µl protein solution + 1 µl reservoir solution) |
4 µl (2 µl protein solution + 2 µl reservoir solution) |
| Volume of reservoir (µl) |
500 |
500 |
| Light conditions |
FR treatment followed by dark conditions and dim 490 nm blue-green LED safelight |
FR treatment followed by dark conditions and dim 490 nm blue-green LED safelight |
