Table 2.

List of 7 primer pairs designed using PrimalScheme.

Name	Pool	Sequence (5ʹ-3ʹ)	Size	GC%	Tm (use 65)
1_LEFT	1	GCTTAGTGCACTCACGCAGT	20	55	61
1_RIGHT	1	ACCGAGCAGCTTCTTCCAAA	20	50	60
2_LEFT	2	AACCACTTACCCGGGTCAGG	20	60	62
2_RIGHT	2	ACTGCAGCAATCAATGGGCA	20	50	61
3_LEFT	1	CATGACACCCCGTGACCTTG	20	60	61
3_RIGHT	1	TGTAGACGTACTGTGGCAGC	20	55	60
4_LEFT	2	AGGGCCAATTCTGCTGTCAA	20	50	60
4_RIGHT	2	ATCAACAGCGGCATGAGAGC	20	55	61
5_LEFT	1	ACGTGAAGTGCTGTCTGACAG	21	52	61
5_RIGHT	1	TTCGCGTGGTTTGCCAAGAT	20	50	61
6_LEFT	2	CTACGGGTACGCTGCTTGTC	20	60	61
6_RIGHT	2	GTATCGTTGCAGTAGCGCGA	20	55	61
7_LEFT	1	GAAATTGACCGCCTCAATGAGG	22	50	61
7_RIGHT	1	CCCATCTGCCTTGTGTGGTC	20	60	61