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Optimized PCR conditions for cDNA amplification to sequence SARS-CoV-2 clinical isolates.
| Temperature | Time | Cycles | |
|---|---|---|---|
| Initial denaturation | 98°C | 30 s | 1 |
| Denaturation | 98°C | 10 s | 40 |
| Annealing and extension | 65°C | 30 s | |
| 72°C | 5 min | ||
| Final extension | 72°C | 5 min | 1 |
| Hold | 4°C | ∞ |
