FIG. 2.

NS5A disrupts PKR dimerization. E. coli AG1688 cells were cotransformed with expression plasmid combinations encoding cI-PKR K296R and GST (column 1), GST-NS5A 1b-wt (column 2), GST-K3L (column 3), or GST-NS1 (column 4), mixed with the indicated dilution of λKH54 phage, and spotted onto plates containing agar medium. Plates were incubated and visually scored for colony formation (dark spots) as described in Materials and Methods. Column 5 contains E. coli harboring cI-Rop and GST-NS5A 1b-wt expression plasmids (control). cI fusion protein dimerization is indicated by colony formation. Shown is a photograph of a plate from a representative experiment.