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Sequences of mutagenic NS5A oligonucleotides
| Construct | Mutagenic primera | Mutation(s) |
|---|---|---|
| NS5A 1b-2 | 5′ GACTCCCCAGATGTTGACCTCATC | A2224V |
| NS5A 1b-5b | ||
| NS5A 1b-4 | 5′ TTGTCTGCGCTTTCCTTGAAGGCAGCATGCACTGGCCGTCACGAC | P2209L, T2214A, T2217G |
Underlined codons correspond to the indicated amino acid mutations.
See Materials and Methods for details on the construction of this construct.
