Table 3.

Differentially abundant proteins (DAP) and actin-related biological pathways in chickens fed xylo-oligosaccharide and arabinoxylan-rich fraction^a

Database	Pathway	P-value	Downregulated (↓)/ upregulated (↑) DAP
Gene Ontology	Actin filament polymerization	0.0000	↓ RAC1, ARPC4, COTL1, VIL1, GSN, CTTN
	Barbed-end actin filament capping	0.0015	↓ HSPB1, VIL1, GSN, CAPZA2
	Actin filament severing	0.0046	↓ VIL1, GSN; ↑ DSTN
	Actin cytoskeleton reorganization	0.0092	↓ RAC1, MYH9, CTTN, EZR, ANXA1
	Actin filament-based movement	0.0191	↓ MYH9, MYO6, MYO1A
	Regulation of cell-cell adhesion mediated by integrin	0.0433	↓ ADA; ↑ DPP4
	Epithelial cell differentiation	0.0499	↓ ANXA4, BDH2, VIL1, HNRNPH3
Reactome	Gap junction degradation	0.0328	↓ MYO6, AP2M1, CLTC
	RHO GTPases activate PAKs	0.0402	↓ RAC1, CDC42, MYLK
	RHO GTPases Activate WASPs and WAVEs	0.0464	↓ RAC1, CDC42, ARPC4, ACTR2

^aXylo-oligosaccharide and arabinoxylan-rich fraction supplemented at 0.5% and 1% level, respectively. RAC1 Ras-related C3 botulinum toxin substrate 1, ARPC4 Actin-related protein 2/3 complex, subunit 4, COTL1 Coactosin-like F-actin binding protein 1, VIL1 Villin 1, GSN Gelsolin, CTTN Cortactin, HSPB1 Heat shock protein family B (small) member 1, CAPZA2 Capping actin protein of muscle Z-line alpha subunit 2, DSTN Destrin, actin depolymerizing factor, MYH9 Myosin, heavy chain 9, non-muscle, EZR Ezrin, ANXA1 Annexin 1, MYO6 Myosin 6, MYO1A Myosin IA, ADA Adenosine deaminase, DPP4 Dipeptidyl peptidase 4, ANXA4 Annexin A4, BDH2 3-Hydroxybutyrate dehydrogenase 2, HNRNPH3 Heterogeneous nuclear ribonucleoprotein H3, AP2M1 Adaptor-related protein complex 2 mu 1 subunit, CLTC Clathrin heavy chain, CDC42 Cell division cycle 42, MYLK Myosin light chain kinase, ACTR2 ARP2 Actin-related protein 2 homolog