TABLE 1.
Cells overexpressing fnx1+ do not inhibit the growth of wild-type cellsa
GFP/fnx1 ratio | Cell density (cells/ml) at:
|
% GFP-expressing cells at 24 h | ||
---|---|---|---|---|
0 h | 13 h | 24 h | ||
1:1 | 1.8 × 106 | 7.2 × 106 | 5.3 × 107 | 97 |
1:10 | 1.3 × 106 | 2.4 × 106 | 8.6 × 106 | 56 |
Cells overexpressing fnx1+ from the REP1-fnx1+ plasmid and cells expressing GFP from the pGFP41 plasmid were mixed in a 1:1 or a 10:1 ratio and incubated in conditions that derepress the promoter. By 24 h the GFP-expressing cells overgrew the fnx1+-expressing cells.