Table 2.
Protein hits identified from Intact Cell CETSA
| PlasmoDB ID | Target name | Heat challenge (°C) | MDT (nM) |
|---|---|---|---|
| PF3D7_0513300 | Purine nucleoside phosphorylase | 60 | 22.23 |
| PF3D7_0110800 | Transcription initiation factor TFIIB putative | 60 | 196.53 |
| PF3D7_0204700 | Hexose transporter | 50 | 316.49 |
| PF3D7_0311300 | Phosphatidylinositol 3- and 4-kinase putative | 60 | 985.85 |
| PF3D7_0312800 | 60S ribosomal protein L26 putative | 50 | 189.79 |
| PF3D7_0407800 | Conserved Plasmodium protein unknown function | 60 | 609.72 |
| PF3D7_0409400 | Chaperone protein DnaJ | 60 | 1605.60 |
| PF3D7_0505500 | DNA mismatch repair protein MSH6 putative | 60 | 383.36 |
| PF3D7_0505700 | Conserved Plasmodium membrane protein unknown function | 60 | 3846.84 |
| PF3D7_0526200 | ADP-ribosylation factor GTPase-activating protein putative | 50 | 1290.89 |
| PF3D7_0611600 | Basal complex transmembrane protein 1 | 60 | 351.65 |
| PF3D7_0710600 | 60S ribosomal protein L34 | 50 | 339.18 |
| PF3D7_0819600 | Conserved Plasmodium protein unknown function | 50 | 1440.99 |
| PF3D7_0822900 | Conserved Plasmodium protein unknown function | 60 | 1137.80 |
| PF3D7_0914700 | Major facilitator superfamily-related transporter putative | 50 | 479.60 |
| PF3D7_0919900 | Regulator of chromosome condensation-PP1-interacting protein | 60 | 620.45 |
| PF3D7_1014900 | Conserved Plasmodium protein unknown function | 60 | 1221.07 |
| PF3D7_1031600 | Conserved Plasmodium protein unknown function | 60 | 19,500.89 |
| PF3D7_1037300 | ADP/ATP transporter on adenylate translocase | 50 | 240.00 |
| PF3D7_1109900 | 60S ribosomal protein L36 | 50 | 150.27 |
| PF3D7_1116800 | Heat shock protein 101 | 50 | 817.34 |
| PF3D7_1121600 | Exported protein 1 | 50 | 982.29 |
| PF3D7_1124700 | GrpE protein homolog mitochondrial putative | 60 | 735.92 |
| PF3D7_1129000 | Spermidine synthase | 50 | 265.77 |
| PF3D7_1134000 | Heat shock protein 70 | 60 | 994.72 |
| PF3D7_1211900 | Non-SERCA-type Ca2+ -transporting P-ATPase | 50 | 430.33 |
| PF3D7_1341300 | 60S ribosomal protein L18-2 putative | 50 | 534.94 |
| PF3D7_1352800 | Vacuolar fusion protein MON1 putative | 60 | 384.28 |
| PF3D7_1360800 | Falcilysin | 60 | 749.91 |
| PF3D7_1417500 | H/ACA ribonucleoprotein complex subunit 4 putative | 60 | 784.24 |
| PF3D7_1431700 | 60S ribosomal protein L14 putative | 50 | 665.11 |
| PF3D7_1434800 | Mitochondrial acidic protein MAM33 putative | 60 | 267.45 |
| PF3D7_1471100 | Exported protein 2 | 50 | 1188.97 |
Analysis was conducted at 50 °C and 60 °C across 1166 and 825 proteins, respectively.