Fig. 2.

Notch signalling-dependent rescue of HOX gene expression in TBXT-depleted NMPs. (A) Scheme depicting the experimental design of the TBXT shRNA-wild-type NMP co-culture experiment. (B,C) qPCR expression analysis of TBXT and HES5 (B) and HOX genes belonging to paralogous groups B and C (C) under the different experimental conditions depicted in A. Error bars represent mean±s.e.m (n=3-6 independent experiments). *P≤0.05, **P≤0.01, ***P≤0.001 {one-sample t-test and Wilcoxon test [TiKD without Tet versus TiKD (+Tet)] or an unpaired, two-tailed t-test [TiKD (+Tet) versus TiKD sorted (+Tet) versus TiKD (+DAPT +Tet)]}. KD, knockdown; WT, wild type.