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PCR (bottom) and Southern blot (top) results for Mycoplasma haemofelis 16S rRNA gene among cats negative for both FIV and FeLV. Lane 1: 100 bp molecular weight marker; Lane 2: Positive DNA control; Lane 3: Negative water control; Lane 4: Negative DNA control; Lanes 5 to 29: Clinical samples tested for M haemofelis DNA among both FIV- and FeLV-negative cats; Lane 30: Previously known positive clinical sample.
