Figure 1.

A screen of 727 FDA-approved drugs identified lansoprazole (LNP) as a potent potentiator of the antifungal activity of amphotericin B (AmB) against C. auris. a) An NIH drug collection library containing 727 drugs was screened at 16 µM in RPMI medium at 35 °C for 24 hr in the presence of 0.125×MIC of AmB (0.25 µg/mL). Growth of C. auris AR0390 was detected by measuring the optical density (OD₆₀₀). The dotted line indicates 80% growth inhibition of C. auris. b) A time-kill assay of AmB at 2 µg/mL (1×MIC), LNP at 40 µg/mL, or a combination of the two drugs in RPMI media against C. auris AR0390 at 35°C for 24 hr. Time-kill assay results are shown as the mean values of CFU ± SD obtained from two independent experiments.