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Two alternative pathways to make AEGIS nucleoside triphosphates
A. Deoxynucleoside dP3 was converted into monophosphate by DmdNKE with ATP as the phosphate donor. Excess ATP and ADP were destroyed by NaIO4 and heating. Blue arrows: substrates; red arrows: products. AdOx: adenosine dialdehyde. B. dP3MP was converted into dP3DP and dP3TP by incubation with ChuPPK2 with PolyP6. Nucleotide products were confirmed by their UV spectra and retention time compared to authentic standards.
