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. Author manuscript; available in PMC: 2024 Mar 4.
Published in final edited form as: ACS Synth Biol. 2023 May 25;12(6):1772–1781. doi: 10.1021/acssynbio.3c00060

Table 2.

Summary of PPK2 activities on AEGIS nucleoside monophosphates*

Substrate DtCs PPK2 ChuPPK2 AaurPPK2
2nd generation ribonucleotide rP2MP di-: 49%
tri: 3%
21%
70%
32%
61%
rX2MP di-: 53%
tri: 10%
24%
71%
21%
51%
rZ2MP di: 32%
tri: 43%
26%
59%
37%
55%
rK2MP di: 66%
tri: 23%
19%
77%
19%
76%
2nd generation deoxy-ribonucleotide dP2MP di: 5%
tri: 2%
8%
3%
3%
2%
dX2MP di: 21%
tri: 3%
18%
32%
3%
6%
dZ2MP di: 17%
tri: 26%
22%
58%
27%
24%
dK2MP di: 26%
tri: 42%
19%
72%
13%
44%
3rd generation deoxy-ribonucleotide dP3MP di: 27%
tri: 4%
19%
27%
19%
47%
dX3MP di: 16%
tri: none
21%
27%
7%
5%
dK3MP di: 25%
tri: 45%
19%
72%
13%
44%
*

By UV absorbance of the respective HPLC peaks. Addition of unreacted monophophate makes the sum 100%.