Fig. 7.
The sensitivity of AcHV2 channels to pHi determined in inside-out patches. (A–D) Families of currents all in the same inside-out patch with pHo 5.5 in the pipette solution were recorded in 10 mV increments up to the voltage indicated. In all cases, Vhold was −40 mV. Pulse duration was shortened for larger depolarizations to minimize proton depletion/accumulation. (E) Current–voltage curves for the families shown in (A–D). Currents were fitted with a single exponential and the extrapolated value or the peak current was plotted. (F) Conductance-voltage relationships for the same families.