Figure 2. Effects of high-fat diet and chemogenetic silencing of ventral hippocampus (vHPC)–nucleus accumbens (NAc) or vHPC–medial prefrontal cortex (mPFC) pathway on c-Fos expression in ventral CA1/subiculum.

(A) Schema of exposure to a novel arena containing two identical and novel objects. (B) Representative images of c-Fos-positive (Fos+) cells in the ventral CA1/subiculum for each group. (C) Quantification of c-Fos+ cells in the ventral CA1/subiculum of the different groups. Data are shown as the number of c-Fos+ cells per mm². The area corresponding to ventral CA1/subiculum area is delineated. Scale bar is set at 500 nm. (D) Representative example of mCherry and c-Fos labelling in the ventral CA1/subiculum. (E) Percentage of mCherry+ and c-Fos+ cells over total number of mCherry+ in the different groups. Scale bar is set to 250 nm and data are shown as a percentage of cells. Each point represents a single animal value. Diet effect: *p < 0.05, **p < 0.01; DREADD effect: ^##p < 0.01 (two-way analysis of variance [ANOVA]).

Figure 2—figure supplement 1. Effect of high-fat diet and chemogenetic manipulation on c-Fos expression in hippocampus, nucleus accumbens (NAc) and medial prefrontal cortex (mPFC).

Quantification of c-Fos-positive cells in the ventral CA3 (A), the NAc (B) and the mPFC (C) of the different groups. Data are shown as the number of c-Fos-positive cells per mm². Diet effect: *p < 0.05, **p < 0.01, ***p < 0.001; DREADD effect: ^##p < 0.01.