Figure 3. Impacts of high-fat diet and chemogenetic silencing of ventral hippocampus (vHPC)–nucleus accumbens (NAc) or vHPC–medial prefrontal cortex (mPFC) pathway on object-based memory.
(A) Schema of object recognition memory (ORM) task (top) and ORM performance expressed as percentage of exploration time of novel (empty bars) or familiar (striped bars) object over both objects (bottom). (B) Schema of object location memory (OLM) task (top) and OLM performance expressed as percentage of exploration time of novel (empty bars) or familiar (striped bars) location over both objects (bottom). Each point represents a single animal value. Diet effect: **p < 0.01 (two-way analysis of variance [ANOVA], diet × pathway). Difference between groups: *p < 0.05, **p < 0.01 (two-way ANOVA, diet × DREADD for each pathway, significant interaction followed by post hoc test). Different from 50%: #p < 0.05 (one-sample t-test).
Figure 3—source data 1. Impacts of high-fat diet and chemogenetic silencing of ventral hippocampus (vHPC)–nucleus accumbens or vHPC–medial prefrontal cortex pathway on object-based memory.
elife-80388-fig3-data1.xlsx (20.2KB, xlsx)
Figure 3—figure supplement 1. Effect of high-fat diet and chemogenetic manipulation on anxiety-like behaviours.
Schema of elevated plus-maze task (A). Results are presented as percentage of open arm time (calculated as open arm time over total time spent in both open and closed arms × 100; B) and percentage of open arm entries (calculated as open arm entries with respect to the total number of entries in both open and closed arms × 100; C).
Figure 3—figure supplement 1—source data 1. Effect of high-fat diet and chemogenetic manipulation on anxiety-like behaviours.
elife-80388-fig3-figsupp1-data1.xlsx (15.1KB, xlsx)