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. 1998 Sep;18(9):5355–5363. doi: 10.1128/mcb.18.9.5355

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Copyright © 1998, American Society for Microbiology

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FIG. 2 — (A) Western blot analysis of the immunoprecipitates using antibodies against CDK8 and hSrb7. Immunoprecipitates (IP) of anti-CDK8 (lane 2), anti-Srb7 (lane 3), and control (lanes 4 and 5) antibodies and 10% of the input protein fraction (lane 1) were analyzed by SDS–5 to 12% PAGE. The Western blots were probed for CBP/p300 and RNAPII (top panel), PCAF (middle panel), and Swi-Snf component BAF47 (bottom panel). (B) Western blot analysis of the immunoprecipitates using antibodies against histone acetyltransferases PCAF and CBP. Immunoprecipitates of anti-CTD (lane 2), anti-PCAF (lane 3), anti-CBP (lane 4), and control (lane 5) antibodies and 10% of the input protein fraction (lane 1) were analyzed by SDS–5 to 12% PAGE and Western blot analysis. The Western blots were probed for CBP/p300 (top panel), PCAF (second panel), RNAPII (third panel), and Swi-Snf component BAF47 (bottom panel).