TABLE 2.

Plasmids used in this study

Plasmid	Description
pRS313-MED6	Wild-type MED6 gene cloned into EcoRI/BamHI sites of pRS313 (HIS3 CEN-ARS)
pRS313-med6ts2	med6-ts2 gene cloned into EcoRI/BamHI sites of pRS313 (HIS3 CEN-ARS)
pRS316-med6ts2	med6-ts2 gene cloned into EcoRI/BamHI sites of pRS316 (URA3 CEN-ARS)
pRS316-M6ES2	SRB4-101 suppressor clone isolated from suppressor genomic library
pRS316-S4SUP	SRB4-101 suppressor clone retrieved from YCL50 by gap repair
pRS313-med6ts1	med6-ts1 gene cloned into EcoRI/BamHI sites of pRS313 (HIS3 CEN-ARS)
pRS313-med6ts6	med6-ts6 gene cloned into EcoRI/BamHI sites of pRS313 (HIS3 CEN-ARS)
pRS313-hMED6	6-histidine-tagged MED6 gene cloned into EcoRI/BamHI sites of pRS313 (HIS3 CEN-ARS)
pEh-RGR1N	N-terminal region of histidine-tagged RGR1 (1–460 amino acids) cloned into NheI/SspI sites of pET3a