Table 3.
The prevalence of the APC: c.835-8 A > G somatic mutation and its association with the intratumoral presence of pks+ E. coli+, pks+ E. coli−, all pks+ bacteria and pks- E. coli+.
| All CRCs | EOCRCs (CRC < 45 yrs) | LOCRCs (CRC ≥ 45 yrs) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Intratumoural bacteria | APC: c.835-8 A > G mutation | APC: c.835-8 A > G wildtype | P valuea | Adjusted Odd Ratio (95% CI)a | APC: c.835-8 A > G mutation | APC: c.835-8 A > G wildtype | P valuea | Adjusted Odd Ratio (95% CI)a | APC: c.835-8 A > G mutation | APC: c.835-8 A > G wildtype | P valuea | Adjusted Odd Ratio (95% CI)a |
| Total CRCs | 58 (3.3%) | 1701 (96.7%) | - | - | 15 (3.3%) | 439 (96.7%) | - | - | 43 (3.3%) | 1262 (96.7%) | - | - |
| pks + E. coli + | 0.0252 | - | 0.0219 | - | 0.1820 | - | ||||||
| Not detected | 41 (3.1%) | 1287 (96.9%) | - | Ref | 9 (2.5%) | 351 (97.5%) | - | Ref | 32 (3.3%) | 936 (96.7%) | - | Ref |
| Detected | 11 (6.3%) | 163 (93.7%) | - | 2.20 (1.05–4.25) | 4 (9.5%) | 38 (90.5%) | - | 4.26 (1.10–14.02) | 7 (5.3%) | 125 (94.7%) | - | 1.78 (0.70–3.94) |
| Unknown | 6 (2.3%) | 251 (97.7%) | - | - | 2 (3.8%) | 50 (96.2%) | - | - | 4 (2%) | 201 (98%) | - | - |
| pks + E. coli− | 0.3570 | - | 0.9931 | - | 0.6098 | - | ||||||
| Not detected | 48 (3.6%) | 1279 (96.4%) | - | Ref | 13 (3.5%) | 357 (96.5%) | - | - | 35 (3.7%) | 922 (96.3%) | - | - |
| Detected | 4 (2.3%) | 171 (97.7%) | - | 0.61 (0.18–1.54) | 0 (0%) | 32 (100%) | - | ntb | 4 (2.8%) | 139 (97.2%) | - | 0.76 (0.22–1.95) |
| Unknown | 6 (2.3%) | 251 (97.7%) | - | - | 2 (3.8%) | 50 (96.2%) | - | - | 4 (2%) | 201 (98%) | - | - |
| pks + (all bacteria) | 0.3775 | - | 0.2537 | - | 0.6323 | - | ||||||
| Not detected | 37 (3.1%) | 1150 (96.9%) | - | Ref | 9 (2.7%) | 327 (97.3%) | - | Ref | 28 (3.3%) | 823 (96.7%) | - | Ref |
| Detected | 15 (4%) | 360 (96%) | - | 1.32 (0.69–2.40) | 4 (5.2%) | 73 (94.8%) | - | 2.03 (0.53–6.54) | 11 (3.7%) | 287 (96.3%) | - | 1.19 (0.56–2.37) |
| Unknown | 6 (3%) | 191 (97%) | - | - | 2 (4.9%) | 39 (95.1%) | - | - | 4 (2.6%) | 152 (97.4%) | - | - |
| pks- E. coli + | 0.1574 | - | 0.8141 | - | 0.0900 | - | ||||||
| Not detected | 47 (3.3%) | 1374 (96.7%) | - | Ref | 12 (3.2%) | 365 (96.8%) | - | Ref | 35 (3.4%) | 1009 (96.6%) | - | Ref |
| Detected | 5 (6.2%) | 76 (93.8%) | - | 1.99 (0.67–4.72) | 1 (4%) | 24 (96%) | - | 1.29 (0.07–7.07) | 4 (7.1%) | 52 (92.9%) | - | 2.56 (0.74–6.82) |
| Unknown | 6 (2.3%) | 251 (97.7%) | - | - | 2 (3.8%) | 50 (96.2%) | - | - | 4 (2%) | 201 (98%) | - | - |
aMultiple logistic regression adjusted for CRC diagnosis age, sex and study cohort. Samples with unknown intratumoural presences and tumour characteristics were excluded in the statistical testing.
bNot tested as deemed non-sensical due to the group containing zero variable.
Statistically significant p < 0.05 values are in bold.