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. 2024 Feb 20;15:1354297. doi: 10.3389/fimmu.2024.1354297

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Copyright © 2024 Masad, Idriss, Mohamed, Al-Sbiei, Bashir, Al-Marzooq, Altahrawi, Fernandez-Cabezudo and Al-Ramadi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lymphocyte activation in lymphoid tissues following MH treatment. BALB/c mice were orally gavaged with either SC or MH for 4 weeks. Following treatment, lymphoid organs were processed for flow cytometry analysis. Cells from MLNs (A–C), ILNs (D–F), and spleens (G–I) were analyzed to quantify the percentage of Sca-1⁺ CD8⁺ T cells (B, E, H), and Sca-1⁺ CD4⁺ T cells (C, F, I). Representative dot plots are shown in (A, D, G). The values for individual mice and mean ± SEM are shown. The data is pooled from 5 (A–C), 4 (D–F), and 3 independent experiments (G–I). p values were calculated using the unpaired Student’s t-test, * (p ≤ 0.05), and ** (p ≤ 0.01).