FIGURE 8.

Co-injection of wnt4 RNA rescues the Prdm15 MO–induced reduced gene expression. (A) Anterior view of stage 13 embryos injected with Prdm15 MO in combination with wnt4 RNA refers to the measured rax and pax6 expression area. Black arrowheads point to the reduced gene expression. Wnt4 RNA could partially rescue the Prdm15 MO–induced reduced marker gene expression. (B) Statistical evaluation of the marker gene expression area of rax and pax6 as described in (A). (C) Dorsal view of stage 13 embryos injected with Prdm15 MO in combination with wnt4 RNA illustrates the measured pax6 expression area. Black arrowhead points to the reduced gene expression. Co-injection of wnt4 RNA restores the Prdm15 MO–induced pax6 expression. (D) Statistical evaluation of the marker gene expression area as described in (C) shows rescue of the dorsal pax6 expression by wnt4 RNA. (E) Anterior and lateral views of stage 20 and stage 23 embryos, respectively, injected with Prdm15 MO in combination with wnt4 RNA illustrate the measured snai2 and foxd3 expression area. Black arrowheads point to the reduced gene expression. Co-injection of wnt4 RNA rescues the Prdm15 MO–induced reduced marker gene expression. (F) Statistical evaluation of snai2 and foxd3 expression area as described in (E) shows a significant rescue of the expression area of snai2 and foxd3. Abbreviations: foxd3, forkhead box D3; GFP, green fluorescent protein; inj., injected; MO, morpholino oligonucleotide; n, number of independent experiments; ns, non-significant; pax6, paired box 6; Prdm15, PR-domain zinc finger protein 15; rax, retina and anterior neural fold homeobox; snai2, snail family transcriptional repressor 2; st., stage; uninj., un-injected; wnt4, wnt family member 4. Error bars indicate standard errors of the means. ns, p > 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001.