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. 2024 Mar 4;7:264. doi: 10.1038/s42003-024-05934-y

Fig. 5. CGRP suppresses TGF-β1 signaling and corneal stromal fibroblast activation in vitro and in vivo.

Fig. 5

Murine corneal fibroblasts were cultured in a medium supplemented with 10 ng/ml TGF-β1 for 24 h, in the presence or absence of 1 µM CGRP. CGRP significantly decreased the TGF-β1-mediated expression of α-SMA assessed via RT-PCR (a), western blot (b), and immunostaining (c) in vitro (n = 3 per group). Corneas derived from CGRP-treated mice showed significantly lower expression of TGF-β1 compared to PBS-treated controls in vivo in RT-PCR (d, n = 4 per group), western blot (e), and immunostaining (f, scale = 50 μm). Similarly, α-SMA levels in RT-PCR (g, n = 4 per group), western blot (h), and immunostaining (f) were significantly elevated post-injury and decreased by CGRP treatment in vivo. The data were presented as mean ± SEM, and the statistical significance was determined by one-way ANOVA with pairwise comparison. *p < 0.05, **p < 0.01, ***P < 0.001, ****P < 0.0001. (Legend in d, g: Black Circle = Naïve, Pink Square = PBS, Blue Triangle = CGRP).