Fig. 2.

Differentiation of collagen gel-embedded OCys cultivated over 14 days in different low serum containing media (FCS, ITS, hi FCS, HS) in the presence and absence of BMP-2 in concentrations between 33,3 ng/mL and 150 ng/mL. A: Fluorescence microscopic images. Cytoskeleton appears green (iFluor488 phalloidin), nuclei appear blue (DAPI) and DMP1 appears red (Alexa546), scale bars represent 100 μm. B: Expression of OCy-marker genes BGLAP, PDPN, RANKL, MEPE, DMP1 and SOST. Diagrams show fold changes compared to 2 % FCS-based medium, ± upper and lower limit. High variances occur while combining three experiments for ITS-based media (grey, each experiment n = 6, n = 18 in total) and two experiments for hi FCS/HS based media (green, each experiment n = 6, n = 12 in total) due to variations between different donors. Therefore, gene expression data of single experiments are shown in Fig. S1. *p < 0.05; **p < 0.01, ***p < 0.001; ****p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)