Table 1.
Primers and probes used for detection of HIV CA-RNA and CA-DNA.
| Primer name | Sequence | Reference |
|---|---|---|
| polFWDpg | TGTACCAGTAAAATTAAAGCCAGGAA | Detection of unspliced CA-RNA (24) |
| polREVpg | TATGGRTTTTCAGGCCCAATT | |
| HIVpol PROBEpg | FAM-TGGATGGBCCAARRGTYAAACARTGGCCATT-TAM | |
| HIV1LTRTaq1 | GCCTCAATAAAGCTTGCCTTGA | detection of CA-DNA and total CA-RNA (23) |
| HIV1LTRTaq2 | GGCGCCACTGCTAGAGATTTT | |
| HIV LTR Probe | FAM-TGTGACTCTGGTAACTAGAGATCCCTCAGAC-TAM | |
| PDH1 | TGAAAGTTATACAAAATTGAGGTCACTGTT | Reference gene for CA-DNA assay (23) |
| PDH2 | TCCACAGCCCTCGACTAACC | |
| PDH Joe/TAMRA | JOE-CCCCCAGATACACTTAAGGGATCAACTCTTAATTGT-TAM | |
| PrimePCR Probe Assay : IPO8, human, Cy5-labeled | Assay ID: qHsaCEP0057620 | reference gene for CA-RNA assay (Bio-Rad) |
| PrimePCR Probe Assay : TBP, human, Hex-labeled, Bio-Rad | Assay ID: qHsaCIP0036255 | reference gene for CA-RNA assay (Bio-Rad) |