FIG. 5.

Effect of a carboxy-terminal deletion of MeCP2 on transcriptional repression of the methylated-leukosialin promoter. Methylated (filled bars) or unmethylated (hatched bars) LS5CAT(−91/+90) construct (4 μg) was cotransfected with pPacSp1 (0.5 μg) and an expression plasmid (0.5 μg) bearing genes encoding various C-terminal deletion mutations of MeCP2 into Drosophila SL2 cells. Amino acid residues of MeCP2 encoded in the mutants are shown in parentheses. A5C is the insertless expression vector. GPA is a control plasmid in which the cDNA of human glycophorin A, an erythroid membrane protein, is cloned into the A5C vector. Relative CAT activities compared with that of unmethylated LS5CAT cotransfected with pPacSp1 and A5C are presented. Values are the means and standard deviations of results from three independent experiments.