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. 2024 Feb 5;12(3):e01365-23. doi: 10.1128/spectrum.01365-23

Fig 3.

Fig 3

Effect of upregulating NTCP on HBV-infected HPCs. Differentiated podocytes were transfected with WT-NTCP by lentiviral plasimd, and the expression of NTCP measured by qPCR was significantly upregulated after transfection with WT-NTCP (A). HPCs in the HBV and HBV + WT-NTCP were incubated with HepG 2.2.15 supernatant (containing HBV) for 16 h, with HPCs incubated with cell culture medium as the control. The levels of HBV cccDNA (B) and HBV DNA (C) from the indicated groups were measured by RT-qPCR. The levels of HBsAg (D) and HBeAg (E) in the supernatant from the indicated groups were detected by enzyme-linked immunosorbent assay. (F, G) Representative images of HBcAg (green) and 4′,6-diamidino-2-phenylindole nuclear (blue) staining in differentiated HPCs. The levels of HBV cccDNA (B), HBV DNA (C), HBsAg (D), HBeAg (E), and HBcAg (F, G) were significantly increased after upregulating NTCP. Scale bar = 200 µm. Results are mean ± SD for three individual experiments. *P < 0.05, **P < 0.01.