Fig 5.

Expression of human podocyte cytoskeletal proteins after HBV infection. Differentiated podocytes were transfected separately with shRNA-NC, shRNA-NTCP, WT-NTCP, and MUT-NTCP by lentiviral plasmid, then incubation with HepG 2.2.15 supernatant for 16 h, and HPCs incubated with cell culture medium as the control. The expressions of nephrin (A), synaptopodin (B), and CD2AP (C) from the indicated groups were detected by western blotting. The levels of nephrin (A), synaptopodin (B), and CD2AP (C) were significantly increased after downregulating, inhibiting, and mutating NTCP. The levels of nephrin (A), synaptopodin (B), and CD2AP (C) were reversed after upregulation of NTCP. In the expression of nephrin (A), synaptopodin (B), and CD2AP (C), GAPDH was repeated as the same internal control experiment. Results are mean ± SD for three individual experiments. *P < 0.05, **P < 0.01.