Fig 8.

Expression levels of SIRT1 in Beas 2B cells. (A) Beas 2B cells were treated with A. baumannii and L-serine. The expression of SIRT1 was detected by immunoblotting, with β-actin used as a loading control. It was quantified and normalized to the β-actin values. (B) Beas 2B cells were treated as described above. The expression of SIRT1 was measured by qRT-PCR. It was quantified and normalized to the GAPDH values. (C) Immunofluorescence staining was performed to observe the expression of SIRT1 (green) in Beas 2B cells, with the nuclei stained with DAPI (blue). Scale bar, 100 μm. Data are presented as the mean ± SD. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001. ^*Compared with the control group.