Extended Data Fig. 4 |. Degradation of validated pomalidomide-sensitive ZF degrons induced by the pomalidomide analogs.

(a) Normalized eGFP intensity in 14 ZF reporter cell lines treated with different doses of 81 pomalidomide analogs ranging from 4.3 nM to 20 μM. Each block of 4.3 nM to 20 μM doses on the x axis represents one ZF reporter cell line. Data are mean of at least 3 independent replicates. (See source data for replicates, mean, SD) (b–d) Box-and-Whisker plots with statistical analysis for pomalidomide analogs arranged in pairs of C4 and C5 modifications such as acylation (b), Suzuki/Sonogashira coupling (c) and effect of -F group (d) on the phthalimide ring. Data are shown for cells treated with 5 μM of each compound (for acylated and Suzuki/Sonogashira couplings) and 20 μM data of each compound for deciphering the -F group effect. For b-d figure panels data points are the mean of at least 3 independent replicates. Centre of the box plot is median. Minima and maxima are 1.5 times the interquartile range over the 75th and under the 25th percentile, respectively.