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. Author manuscript; available in PMC: 2024 Mar 5.
Published in final edited form as: Nat Chem. 2023 Dec 18;16(2):218–228. doi: 10.1038/s41557-023-01379-8

Extended Data Fig. 1 |. Establishment and validation of automated imaging assay for the degradation of ZFs.

Extended Data Fig. 1 |

a) Representative readouts of the imaging assay in 384-well plates, demonstrating robust detection of ZF-tagged eGFP degradation as induced by pomalidomide. Shown are images of U2OS cells with stable expression of pomalidomide-sensitive ZFP91 degron reporter. Z′ value was calculated using an in-built module in the Harmony software (See materials and methods). b, c) Immunoblots (from at least two independent replicates) quantifying off-target degradation of endogenous ZF proteins ZFP91 by MS4078 (ALK PROTAC) in a dose-dependent manner across cell lines SU-DHL-1 (b) and H2228 (c). d) Label-free proteomic analysis of MS4078 in MOLT4 cells.