TABLE 3.
Increased methylation accessibility of the mat2P locus in the rhp6−/sng1− mutant depends on the switching competence of the strain
| Genotype | % Methylationa
|
|||
|---|---|---|---|---|
| Dimethylation (DpnI)
|
Hemi- + dimethylation (BclI), site b | |||
| Site a | Site bb | Sites a + b | ||
| h90 | 0.0 | 0.0 | 0.0 | 0.9 |
| h90 swi6− | 23.8 | 10.0 | 33.8 | 25.5 |
| Msmto swi6− | 31.2c | 8.9c | 40.1c | ND |
| h90 rhp6−/sng1− | 17.4 | 7.5 | 24.9 | 13.5 |
| Msmto rhp6−/sng1− | 1.5 | 1.6 | 3.1 | 8.3 |
a
Quantitation by densitometry of the autoradiograph shown in Fig. 6B.
b
The area of the band comigrating at the position of site b in the control lane in Fig. 6B was quantitated and subtracted from the areas determined for band b in lanes 1 to 4. ND, not determined.
c
Data from Fig. 8 were quantitated.