TABLE 4.
Increased methylation accessibility of the mat3M locus in the rhp6−/sng1− mutant is also switching dependent
| Genotype | % Methylationa
|
||||
|---|---|---|---|---|---|
| Hemi- + dimethylation (BclI lane), sites a + b + cb | Dimethylation (DpnI lane)
|
||||
| Site a | Site bb | Site c | Sites a + b + cb | ||
| h90 | 26.7 | 0.0 | 0.0 | 0.0 | 0.0 |
| h90 swi6− | 50.2 | 4.9 | 5.9 | 17.1 | 27.9 |
| Msmto swi6− | ND | 5.9c | 5.0c | 19.6c | 30.5c |
| h90 rhp6−/sng1− | 51.8 | 4.1 | 5.5 | 16.8 | 26.4 |
| Msmto rhp6−/sng1− | 55.2 | ∼0.2 | ∼0.4 | ∼0.4 | ∼1.0 |
a
Quantitation by densitometry of the autoradiograph shown in Fig. 8B.
b
For quantitation, the contribution of the slower-migrating band at 2.7 kb in Fig. 7B (lanes 2 and 3), indicated by the arrowhead, was also included. This site is not indicated in the map in Fig. 7 based on published sequence and may represent a polymorphic site. ND, not determined.
c
Data from Fig. 8 were quantitated.