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. 2024 Mar 5;25:245. doi: 10.1186/s12864-024-10150-3

Table 5.

Gene ontology showing enriched biological processes, molecular functions, and cellular components of hepatic innate and adaptive immune genes in low- compared with high-RFI steers1

Gene (s) Raw P-value FDR2
GO biological process
Positive regulation of immunoglobulin production IL13, IL6, TBX21, IL4, IL 1.23E-09 2.42E-07
Positive regulation of interleukin-10 production IL13, IL6, CD40LG, IL4, 3.75E-08 5.62E-06
T-helper cell differentiation GATA3, IL6, TBX21, IL4 5.39E-08 7.68E-06
Macrophage activation IL13, IFNG, IL4, TNF 9.23E-08 1.19E-05
Positive regulation of interleukin-13 production GATA3, IL4 1.67E-07 1.96E-05
Vascular endothelial growth factor production TNF, IL6, IFN 2.22E-07 2.46E-05
Microglial cell activation IFNG, IL4, TNF 5.63E-07 5.55E-05
Positive regulation of cytokine production involved in inflammatory response IL6, TNF, IL17A 2.30E-06 1.80E-04
Regulation of acute inflammatory response IL6, IL4, TNF 2.92E-06 2.19E-04
Regulation of complement-dependent cytotoxicity IL13, IL4 6.31E-06 4.07E-04
Wnt signaling pathway involved in kidney development GATA3 1.05E-05 6.22E-04
Positive regulation of vitamin metabolic process IFNG, TNF 1.05E-05 6.36E-04
Detection of lipopolysaccharide LY96 2.94E-05 1.50E-03
Positive regulation of mast cell activation involved in immune response IFNG, TNF 6.91E-05 2.96E-03
Positive regulation of interleukin-23 production GATA3 3.78E-05 1.82E-03
Positive regulation of interleukin-5 production GATA3 3.78E-05 1.82E-03
Positive regulation of isotype switching to IgG isotypes TBX21, IL4 4.72E-05 2.17E-03
GO molecular function
Tumor necrosis factor receptor binding TNF, CD40LG, 3.51E-10 1.97E-07
CD40 receptor binding CD40LG 3.54E-08 1.59E-05
CXCR chemokine receptor binding CXCL8, CXCL10 9.73E-07 3.65E-04
Interleukin-8 receptor binding CXCL8, 6.31E-06 2.18E-03
Toll-like receptor 4 binding LY96 1.58E-05 4.73E-03
Interleukin-2 receptor binding GATA3, IL2 2.20E-05 5.83E-03
Lipopolysaccharide immune receptor activity LY96 2.94E-05 7.34E-03
Toll-like receptor binding LY96 9.51E-05 2.14E-02
GO cellular component
Lipopolysaccharide receptor complex LY96 3.78E-05 1.77E-02

1Low-RFI = feed-efficient beef steers, high-RFI = feed inefficient beef steers

2False discovery rate (FDR; relative to high-RFI)