. 2024 Jan 31;12(3):e03710-23. doi: 10.1128/spectrum.03710-23

TABLE 2.

Biochemical characteristics and clear zone ratio (mm) (mean ± SD) of selected bacterial isolates from Ethiopian hot springs^a

No.	Hot spring	Sample source	Species	Strain	Oxidase	Citrate	Indole	Amylase	Protease	Cellulase	Lipase
1	Woliso	Water	Bacillus cereus	JUW12	+	−	−	1.63 ± 0.2^j	2.8 ± 0.3^f	2.72 ± 0.15^f	1.66 ± 0.15^gh
2	Woliso	Water	Paenibacillus thiaminolyticus	JUW28	+	−	+	1.42 ± 0.16^j	1.58 ± 0.08^gh	3.33 ± 0.17^cdef	3.51 ± 0.24^cd
3	Woliso	Water	Paenibacillus thiaminolyticus	JUW29	+	−	−	3.61 ± 0.36^cdefgh	1.57 ± 0.21^gh	3.81 ± 0.37^abcd	1.59 ± 0.14^h
4	Woliso	Water	Bacillus licheniformis	JUW38	+	−	−	3.79 ± 0.19^cdefg	1.66 ± 0.21^gh	3.83 ± 0.44^abcd	1.72 ± 0.1^gh
5	Woliso	Water	Paenibacillus thiaminolyticus	JUW43	−	−	−	4.09 ± 0.44^bcde	1.6 ± 0.23^gh	2.96 ± 0.51^ef	3.74 ± 1.22^bcd
6	Woliso	Water	Paenibacillus dendritiformis	JUW86	+	−	+	3.76 ± 0.47^cdefg	3.67 ± 0.52^cde	1.4 ± 0.3^g	1.5 ± 0.3^h
7	Woliso	Water	Paenibacillus thiaminolyticus	JUW103	−	−	−	4.92 ± 1.13^a	1.38 ± 0.14^h	1.64 ± 0.2^g	4.47 ± 0.97^ab
8	Woliso	Sediment	Bacillus licheniformis	JUW54	+	−	−	4.3 ± 0.75^abc	3.57 ± 0.6^cde	1.51 ± 0.14^g	1.63 ± 0.24^h
9	Woliso	Sediment	Bacillus licheniformis	JUW62	+	+	−	1.41 ± 0.26^j	1.5 ± 0^jh	3.93 ± 0.7^abc	4.13 ± 0.36^abc
10	Woliso	Sediment	Paenibacillus thiaminolyticus	JUW64	+	−	−	1.44 ± 0.16^j	3.4 ± 0.57^def	1.53 ± 0.29^g	3.64 ± 0.2^cd
11	Wondo G.	Water	Bacillus licheniformis	JUWG3	+	+	−	1.68 ± 0.3^j	1.44 ± 0.31^h	1.43 ± 0.35^g	3.83 ± 0.43^abcd
12	Wondo G.	Water	Bacillus licheniformis	JUWG7	+	−	−	1.38 ± 0.13^j	3.63 ± 0.63^cde	1.73 ± 0.2^g	1.52 ± 0.17^h
13	Wondo G.	Water	Bacillus licheniformis	JUWG8	+	−	−	1.65 ± 0.08^j	1.5 ± 0.07^gh	1.62 ± 0.28^g	3.27 ± 0.15^de
14	Wondo G.	Water	Paenibacillus thiaminolyticus	JUWG9	+	−	−	1.65 ± 0.28^j	1.61 ± 0.11^gh	1.63 ± 0.23^g	4.52 ± 0.41^a
15	Wondo G.	Water	Bacillus licheniformis	JUWG21	+	−	−	3.98 ± 0.42^bcdef	1.59 ± 0.35^gh	1.46 ± 0.23^g	3.44 ± 0.51^cd
16	Wondo G.	Water	Paenibacillus thiaminolyticus	JUWG22	+	−	−	3.14 ± 0.25^ghi	2.19 ± 0.07^g	1.39 ± 0.18^g	3.33 ± 0.48^cde
17	Wondo G.	Water	Bacillus licheniformis	JUWG28	+	−	−	3.27 ± 0.29^fghi	1.7 ± 0.47^gh	3.74 ± 0.94^abcd	1.37 ± 0.1^h
28	Wondo G.	Water	Bacillus licheniformis	JUWG44	+	−	−	1.71 ± 0.24^j	1.88 ± 0.4^gh	4.35 ± 0.63^a	1.32 ± 0.1^h
19	Wondo G.	Water	Bacillus licheniformis	JUWG45	+	−	−	2.85 ± 0.17ⁱ	1.66 ± 0.15^gh	1.4 ± 0.13^g	3.47 ± 0.32^cd
20	Wondo G.	Water	Paenibacillus thiaminolyticus	JUWG50	+	−	−	1.5 ± 0.07^j	1.66 ± 0.15^gh	3.56 ± 0.51^bcde	2.45 ± 0.24^fg
21	Wondo G.	Water	Bacillus licheniformis	JUWG51	−	−	−	1.47 ± 0.32^j	1.96 ± 0.18^gh	1.45 ± 0.21^g	3.46 ± 0.36^cd
22	Wondo G.	Water	Bacillus licheniformis	JUWG53	+	−	−	1.64 ± 0.19^j	1.45 ± 0.21^h	1.71 ± 0.37^g	3.76 ± 1.08^bcd
23	Wondo G.	Water	Paenibacillus dendritiformis	JUWG60	+	−	−	1.85 ± 0.33^j	1.77 ± 0.07^gh	3.67 ± 0.08^abcde	1.5 ± 0.22^h
24	Wondo G.	Water	Bacillus licheniformis	JUWG77	+	−	−	1.92 ± 0.2^j	4.02 ± 0.42^bcd	4.1 ± 0.53^ab	1.44 ± 0.17^h
25	Wondo G.	Water	Bacillus licheniformis	JUWG84	−	−	−	3.88 ± 0.55^bcdefg	2.81 ± 0.35^f	1.25 ± 0^g	1.6 ± 0.31^h
26	Shalla	Water	Bacillus licheniformis	JUS27	+	−	−	1.72 ± 0.1^j	3.8 ± 0.42^cde	1.68 ± 0.31^g	1.75 ± 0.07^gh
27	Shalla	Water	Bacillus licheniformis	JUS50	−	−	−	3.42 ± 0.36^efghi	1.56 ± 0.12^gh	3.47 ± 0.6^bcde	1.66 ± 0.21^gh
28	Shalla	Sediment	Bacillus licheniformis	JUS2	+	−	−	4.58 ± 0.73^ab	1.89 ± 0.49^gh	1.45 ± 0.21^g	3.24 ± 0.45^de
29	Shalla	Sediment	Bacillus licheniformis	JUS3	+	−	−	3.47 ± 0.19^defghi	4.19 ± 0.46^bc	3.51 ± 0.55^bcde	1.36 ± 0.12^h
30	Shalla	Sediment	Bacillus licheniformis	JUS4	−	−	−	1.73 ± 0.14^j	4.65 ± 0.32^ab	1.45 ± 0.21^g	2.6 ± 0.24^ef
31	Shalla	Sediment	Bacillus licheniformis	JUS5	−	−	−	3.62 ± 0.52^cdefg	3.71 ± 0.14^cde	3.33 ± 0.08^cdef	1.6 ± 0.31^h
32	Shalla	Sediment	Bacillus licheniformis	JUS9	+	−	−	1.6 ± 0.31^j	1.59 ± 0.23^gh	1.81 ± 0.32^g	3.86 ± 0.25^abcd
33	Shalla	Sediment	Bacillus licheniformis	JUS10	+	+	−	1.51 ± 0.11^j	1.51 ± 0.38^gh	3.32 ± 0.33^cdef	1.6 ± 0.35^h
34	Shalla	Sediment	Bacillus licheniformis	JUS14	+	−	−	3.91 ± 0.56^bcdefg	4.11 ± 0.43^bc	1.59 ± 0.14^g	1.39 ± 0.28^h
35	Shalla	Sediment	Paenibacillus thiaminolyticus	JUS28	+	−	−	1.72 ± 0.1^j	3.8 ± 0.42^cde	1.68 ± 0.31^g	1.75 ± 0.07^gh
36	Shalla	Sediment	Bacillus licheniformis	JUS33	+	−	−	1.81 ± 0.34^j	1.59 ± 0.22^gh	1.45 ± 0.21^g	3.33 ± 0.33^cde
37	Shalla	Sediment	Bacillus licheniformis	JUS38	+	−	−	2.96 ± 0.31^hi	1.51 ± 0.14^jh	1.24 ± 0.12^g	1.38 ± 0.14^h
38	Shalla	Sediment	Paenibacillus thiaminolyticus	JUS39	+	−	−	1.75 ± 0.23^j	3.24 ± 0.55^ef	3.38 ± 0.54^cdef	1.44 ± 0.25^h
39	Shalla	Sediment	Brevibacillus borstelensis	JUS42	+	−	−	1.6 ± 0.31^j	1.48 ± 0.08^gh	1.81 ± 0.22^g	3.61 ± 0.35^cd
40	Shalla	Sediment	Bacillus licheniformis	JUS49	+	+	−	4.22 ± 0.91^abcd	4.07 ± 0.81^bcd	1.44 ± 0.48^g	1.96 ± 0.7^fgh
41	Shalla	Sediment	Bacillus licheniformis	JUS51	−	−	−	3.79 ± 0.44^cdefg	4.86 ± 0.48^a	1.75 ± 0.4^g	1.69 ± 0.39^gh
42	Shalla	Sediment	Paenibacillus thiaminolyticus	JUS52	+	−	−	3.32 ± 0.33^efghi	1.38 ± 0.14^h	3.14 ± 0^def	1.9 ± 0.16^fgh
43	Shalla	Sediment	Bacillus licheniformis	JUS53	+	−	−	3.8 ± 0.42^cdefg	1.33 ± 0.2^h	3.2 ± 0.35^def	3.68 ± 0.67^cd
44	Shalla	Sediment	Bacillus licheniformis	JUS54	+	−	−	1.51 ± 0.24^j	3.62 ± 0.52^cde	3.72 ± 0.1^abcd	1.7 ± 0.31^gh
45	Shalla	Sediment	Bacillus licheniformis	JUS55	+	−	−	1.51 ± 0.14^j	3.67 ± 0.63^cde	3.98 ± 0.54^abc	1.75 ± 0.29^gh

^{^a}

Initials used to label isolates were based on initials of the hosting university, JU (Jimma University), followed by initials of the study sites, W (Woliso), WG (Wondo Genet), or S (Shalla). Mean values within the column followed by the same letter are not significantly different (P ≥ 0.05).