TABLE 2.
Results of real-time PCR analysis in bacteria-spiked plasma
| Bacteria | Concn (CFUa/mL) | Without incubation | 24-h incubation | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Without bacterial enrichment | With bacterial enrichment | Mean Ct differencea | Without bacterial enrichment | With bacterial enrichment | Mean Ct difference | ||||||||||
| Positive rate |
Ct | Positive rate |
Ct | Positive rate |
Ct | Positive rate | Ct | ||||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | ||||||||
| S. aureus | 104 | 2/5 | 36.62 | 0.68 | 3/5 | 34.79 | 1.15 | 1.83 | 5/5 | 25.45 | 3.57 | 5/5 | 18.17 | 0.95 | 7.28 |
| 103 | 0/5 | NAb | NA | 3/5 | 35.33 | 2.15 | NA | 5/5 | 27.20 | 3.75 | 5/5 | 20.04 | 1.21 | 7.16 | |
| 102 | 0/5 | NA | NA | 1/5 | 37.42 | NA | NA | 4/5 | 27.06 | 5.73 | 5/5 | 22.65 | 1.21 | 4.42 | |
| 101 | 0/5 | NA | NA | 0/5 | NA | NA | NA | 5/5 | 29.24 | 4.59 | 5/5 | 27.35 | 1.25 | 1.89 | |
| B. cereus | 104 | 1/5 | 27.71 | NA | 5/5 | 31.42 | 2.48 | −3.72 | 4/5 | 29.48 | 3.34 | 5/5 | 21.94 | 1.11 | 7.53 |
| 103 | 0/5 | NA | NA | 5/5 | 34.26 | 2.10 | NA | 3/5 | 30.73 | 5.06 | 5/5 | 23.34 | 1.85 | 7.39 | |
| 102 | 0/5 | NA | NA | 2/5 | 34.08 | 3.44 | NA | 0/5 | NA | NA | 5/5 | 24.27 | 2.01 | NA | |
| 101 | 0/5 | NA | NA | 1/5 | 37.36 | NA | NA | 0/5 | NA | NA | 5/5 | 24.72 | 1.04 | NA | |
| E. coli | 104 | 0/5 | NA | NA | 5/5 | 33.15 | 1.03 | NA | 2/5 | 34.89 | 2.15 | 5/5 | 28.41 | 1.91 | 6.48 |
| 103 | 2/5 | 36.79 | 1.56 | 3/5 | 36.22 | 1.73 | 0.56 | 1/5 | 33.25 | NA | 5/5 | 31.88 | 1.26 | 1.38 | |
| 102 | 0/5 | NA | NA | 0/5 | NA | NA | NA | 0/5 | NA | NA | 5/5 | 34.91 | 0.59 | NA | |
| 101 | 0/5 | NA | NA | 0/5 | NA | NA | NA | 0/5 | NA | NA | 1/5 | 36.53 | NA | NA | |
| K. pneumoniae | 104 | 1/5 | 37.12 | NA | 5/5 | 29.63 | 1.34 | 7.49 | 2/5 | 33.40 | 2.06 | 5/5 | 26.78 | 1.20 | 6.62 |
| 103 | 0/5 | NA | NA | 4/5 | 32.70 | 0.54 | NA | 0/5 | NA | NA | 5/5 | 30.78 | 1.61 | NA | |
| 102 | 0/5 | NA | NA | 5/5 | 34.26 | 1.09 | NA | 0/5 | NA | NA | 4/5 | 34.16 | 0.91 | NA | |
| 101 | 0/5 | NA | NA | 2/5 | 34.18 | 0.21 | NA | 0/5 | NA | NA | 0/5 | NA | NA | NA | |
a
Mean Ct difference, difference of Ct mean between with bacterial enrichment and without bacterial enrichment.
b
NA, not applicable due to the result of not detected or lack of data.