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. 2023 Nov 29;149(10):774–787. doi: 10.1161/CIRCULATIONAHA.123.065959

Figure 4.

Figure 4.

Quantification of total HDL and HDL subspecies in LCAT-deficient (-/-), LCAT-heterozygous (+/-), and control (+/+) subjects. A, Representative size and concentration profiles of HDL isolated from LCAT-deficient (-/-), LCAT-heterozygous (+/-), and control (LCAT+/+) subjects. B through F, HDL isolated by ultracentrifugation from plasma (d=1.063–1.21 g/mL) was analyzed by calibrated IMA. The mean HDL subspecies sizes were as follows: extra-small HDL (XS-HDL), 7.8 nm; small HDL (S-HDL), 8.4 nm; medium HDL (M-HDL), 9.2 nm; and large HDL (L-HDL), 10.9 nm. HDL isolated from plasma by ultracentrifugation was subjected to calibrated IMA. The number of subjects was as follows: LCAT+/+, n=14; LCAT+/-, n=6; and LCAT-/-, n=6. P value, 1-way ANOVA with Tukey-Kramer post-tests. ***P<0.001, **P<0.01, and *P<0.05. arb indicates arbitrary units; HDL, high-density lipoprotein; HDL-P, HDL particle concentration determined by calibrated IMA; IMA, ion mobility analysis; and LCAT, lecithin-cholesterol acyltransferase.